Bio-Reveal: THE IDEAL RAPID BIO-DETECTION TOOL
Bio-Reveal is a state of the art, real-time, biological detection system
designed to provide instant results for bio-contamination testing
on surfaces or in liquids. Bio-Reveal applications are diverse and
range from providing users with the instant capability of testing
for mold and bacteria on surfaces as part of water damage loss evaluations
(IICRC S500 and S520), IAQ and industrial hygiene evaluations, bacteria
outbreak investigations as well as being an ideal tool for performing
Post Remediation Verification (PRV) testing for mold and bacteria
remediation projects. Bio-Reveal is an extremely sensitive detection
system and can indicate the presence of not only dangerous mold and
bacterial organisms but somatic materials and bio-films as well,
which are also problematic and are not addressed or detected through
traditional environmental testing methods, such as using culturing
or air sampling techniques.
The accuracy and sensitivity of the Bio-Reveal system make it an ideal
tool for mold and bacteria testing, healthcare infection control and
environmental services monitoring as well as providing the needed bio-contamination
data for ensuring quality assurance, Good Manufacturing Practice (GMP)
and risk management for the food service and food manufacturing industries.
Bio-Reveal is the needed tool for any environmental investigator or contractor
interested in providing their customers with immediate feedback and decision-making
capability on how to deal with a contamination or remediation issue.
The Bio-Reveal system will improve the sales lead generation and sales
closing process for projects, improve overall project turnaround time
and reduce costs significantly. The ability to obtain instant results
and perform testing for less than 10% of traditional methods is the competitive
advantage the Bio-Reveal system offers its users.
The Bio-Reveal luminometer is a handheld device weighing approximately
8-oz. The Bio-Reveal self-contained collection swabs are approximately
6.75 inches in length and are extremely robust, temperature tolerant,
aseptic and produced in an FDA certified facility.
= QUANTIFYING LIFE'S COMMON THREAD
The Bio-Reveal real-time biological detection system measures the quantity
of ATP [adenosine triphosphate] molecules, which are found in all living
organisms such as mold, bacteria, plant and animal cells. Biological
and bio-film residues contain ATP, which can be collected, tested,
and quantified using the Bio-Reveal system.
The Bio-Reveal system uses bioluminescence to detect the presence of
the ATP molecule:
The ATP molecule is the chemical compound
in which energy is stored in all living cells. In the ATP-luminometric
test, the firefly enzyme,
luciferase, [in the presence of the sampled substrate, luciferin, oxygen,
and magnesium ions] catalyzes the conversion of ATP into light through
an oxidation-reduction reaction. The light generated in this biochemical
reaction is directly proportional the amount of ATP (bio-contamination)
present, thus, the light units can be used to generate a relatively
accurate estimate of the total biomass of cells in a sample.
The biochemical reaction is specific for ATP and very efficient, with
almost every molecule of ATP causing the emission of one photon of green
light. As all organisms rely on the ATP molecule as the main carrier
of metabolic energy, it is a very useful indicator of the presence of
living organic cells.
THE Bio-Reveal PROCESS: SIMPLE AND EFFECTIVE
Quantification of intercellular microbial ATP can be conveniently accomplished
using the Bio-Reveal system. The rapid and simplified extraction and
assay procedures allow the user to simply swab the desired surface
[or collect a liquid or water sample by dipping the swab tip for 3
seconds], reassemble the swab tube device and break the snap valve,
which releases the reagent that reacts with the ATP (bio-contamination)
in the sample. The swab is placed into the luminometer and
the readout is displayed in a numerical value measured in relative
light units [RLUs]. By approximating the number of microorganisms
for generating known RLUs, one can estimate the number of microorganisms
in the sample.
REVEALING MORE THAN GERMS: Seeing the BIG PICTURE
The Bio-Reveal system is ideal at detecting total viable mold and bacteria
counts but it can also be used as a sanitation or hygiene check, including
the verification of the presence of somatic cells on a surface. Detecting
organic residues containing ATP allows for a wide variety of uses. The
Bio-Reveal system can detect blood and other body fluids as well as food
residues [not just the microorganisms detected by traditional microbiology
tests]. This total biological test is a much more effective measure of
whether a surface has been effectively cleaned and sanitized.